Xanthoproteic Test for Aromatic Amino Acids

The xanthoproteic test is a chemical test that is used to identify the presence of certain amino acids in a sample. The test involves the us...

Solubility Tests for Amino Acids and Proteins

The solubility of amino acids and proteins is largely dependent on the pH of the solution. The structural changes that occur at different pH...

Osazone Test or Phenylhydrazine Test

This test is used to distinguish between lactose and maltose. The organic compound phenylhydrazine reacts to form osazones with the carbonyl...

Mucic Acid Test for Galactose

This test is highly specific to galactose, which is either present in solutions independently or obtained by lactose hydrolysis. On heating ...

Bial's Test for Pentoses

This test is specific to pentoses and to pentose-containing compounds and is therefore useful for pentose sugar determination. The reaction ...

Seliwanoff’s Test for the Differentiation of Aldoses from Ketoses

This test is a keto hexose-specific timed colour reaction. It is therefore used for the differentiation of aldoses from ketoses. Dehydration...

Picric Acid Test for Sugar Reduction Detection

This is another test for sugar reduction detection. To form a red colored Picramic Acid, the decreasing sugars react with Picric Acid. Reage...

Barfoed's Test to Differentiate Between Mono and Disaccharide Reduction

This test is performed to differentiate between mono- and disaccharide reduction. Monosaccharides are more reactive reducing agents than dis...

Benedict's Test : Reaction, Reagents, and Procedure

Benedict modified the Fehling solution to produce a fairly stable, enhanced single reagent. As a chelating agent, Sodium Citrate works. It i...

Polysaccharide iodine test

Polysaccharide iodine test is performed to differentiate polysaccharides from mono and disaccharides. Principle With various polysaccharide...

Anthrone Test : General Test for Carbohydrates

Another general test for carbohydrates is anthrone reaction. Its  principle is the same as that of Molisch's, except that furfurals and ...

Molisch's Test

The response is based on the fact that H2SO4 concentrated catalyzes the dehydration of sugars to form furfural (from pentoses) or hydroxymet...

Biuret's Test for Protein

Biuret’s test is used to indicate the presence of peptide bond in a substance being tested. The presence of the peptide bond indicates the p...

Ninhydrin's test for Amino Acids

Ninhydrin’s test is used to indicate the presence of amino acids in a substance. Ninhydrin solution is used to detect all types of amino aci...

Pauly's Test for Histidine and Tyrosine

Pauly's test is a chemical test to detect the amino acid histidine and tyrosine. The basic principle in pauly's test is diazotized. ...

Seliwanoff's Test for Ketose Sugars

Seliwanoff's test used to distinguish sugars that included ketose or aldose. Aldose sugars have an aldehyde group, while ketose have a k...

Benedict’s Test for Monosaccharides and Reducing Sugars

Benedict’s test used to indicate the presence of monosaccharides and reducing sugars. Copper sulphate in the benedict’s reagent will react w...

Molisch's Test for Carbohydrates

Molisch's test is used to indicate the presence of carbohydrates in a solution. Molish test applies to all classes of carbohydrates. Nei...

Barfoed's Test for Monosaccharides

Barfoed's test used to distinguish the monosaccharide and disaccharide. Monosaccharides oxidized by Cu 2+ ions form a carboxylic group a...