Picric Acid Test for Sugar Reduction Detection

This is another test for sugar reduction detection. To form a red colored Picramic Acid, the decreasing sugars react with Picric Acid.

Reagents

Picric acid saturation:

• Dissolve 13 g of picric acid in 100 mL of DW, boil and cool.
• Sodium Carbonate (10% solution).

Procedure

Add 1 mL of the above-mentioned reagent to 1 mL of the test solution, followed by 0,5 mL of the 10% solution of sodium carbonate. In a boiling water bath, heat the test tube. The presence of sugar reduction in the solution indicates the appearance of a red color.