Rabu, 22 Februari 2023

Picric Acid Test for Sugar Reduction Detection

This is another test for sugar reduction detection. To form a red colored Picramic Acid, the decreasing sugars react with Picric Acid.

Picric acid reaction


Picric acid saturation:

  • Dissolve 13 g of picric acid in 100 mL of DW, boil and cool.
  • Sodium Carbonate (10% solution).


Add 1 mL of the above-mentioned reagent to 1 mL of the test solution, followed by 0,5 mL of the 10% solution of sodium carbonate. In a boiling water bath, heat the test tube. The presence of sugar reduction in the solution indicates the appearance of a red color.

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