This test is specific to pentoses and to pentose-containing compounds and is therefore useful for pentose sugar determination. The reaction is due to the formation of furfural in the acid medium which in the presence of ferric ions, condenses with orcinol to give a blue-green complex.
|Bial's test result|
Dissolve 1.5g of orcinol in 100 mL of Conc HCL and add 20 drops of 10% solution of Ferric Chloride. Make yourself fresh.
- Add 4 to 5 drops of the test solution to about 2 mL of Bial's reagent.
- Heat until bubbles of gas rise to the surface in a boiling water bath.
- The presence of pentose sugar indicates the formation of the blue-green solution and the precipitate.