Sabtu, 18 Februari 2023

Anthrone Test : General Test for Carbohydrates

Another general test for carbohydrates is anthrone reaction. Its 

principle is the same as that of Molisch's, except that furfurals and hydroxyl-methyl furfurals offer bluish green condensation products with anthrone. 

Anthrone reaction


Anthrone reagent: in conc.H2SO4, 0.2% (w/v) solution. 


In a test tube, add about 2 mL of Anthrone reagent to approximately 0.5-1mL of the test solution and mix thoroughly. 

Watch if the colour changes to bluish green. If not, after keeping them in a boiling water bath for ten minutes, re-examine the tubes. 

A positive test indicates a blue-green color.

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